BME 23L Foundations of Design and Experimentation in Molecular Biology, Part II

Laboratory-based course extending the wet lab skills developed in the first two quarters of the BME 2x series with bioinformatics skills that form the foundation of biomolecular engineering. Focuses on sequencing of low-diversity environmental samples, including: field sampling, DNA extraction from those samples, library preparation, hands-on sequencing using a Nanopore device, base-calling of the raw data, assembly of contigs, quality assessment, contig assignment to species/bins, automated annotation of a single assemblage from collection genome publication.